There’s nothing crabby about RNA sequencing – Researchers have utilized the neural networks of crabs to validate the use of RNA sequencing for the identification of single neurons. @ ´´RNA is an acronym for ribonucleic acid, a nucleic acid. Many different kinds are now known.[1] RNA is physically different from DNA: DNA contains two intercoiled strands, but RNA only contains one single strand. RNA also contains different bases from DNA.´´The main function of RNA is to carry information of amino acid sequence from the genes to where proteins are assembled on ribosomes in the cytoplasm. This is done by messenger RNA (mRNA). A single strand of DNA is the blueprint for the mRNA which is transcribed from that DNA strand.´´

https://simple.wikipedia.org/wiki/RNA

https://www.linkedin.com/company/biotechniques/ https://www.biotechniques.com/cell-and-development-biology/theres-nothing-crabby-about-rna-sequencing/?fbclid=IwAR3sx2GNt04l3WFYubTo6SyVJU3nXN-EdTQIGHBB-4asbDnm69xd67xNFKE

BioTechniques
close-icon
search-icon
  •  
  •  
  •  
  •  
  •  

Categories // Cell development biology //

There’s nothing crabby about RNA sequencing

10 DEC 2019CELL DEVELOPMENT BIOLOGY

Crab Crab

Researchers have utilized the neural networks of crabs to validate the use of RNA sequencing for the identification of single neurons.

A team of researchers, led by David Schulz (University of Missouri, MO, USA), have demonstrated the ‘clawsome’ power of RNA sequencing for the anatomical and functional identification of single neurons.

The use of RNA sequencing is widespread for the identification of cells in the nervous system. However, previous classification studies have been impeded by other features of the cells. This work aimed to validate the accuracy of single-cell transcriptional profiling for the identification of individual neurons.

Speaking to BioTechniques, Schulz commented, “RNA sequencing is, and will be, used not only to understand how neurons work under typical conditions – and what makes them distinct from one another – but also to shed light on how development, growth, learning, injury, and disease change neurons over the lifetime of an individual.”

RNA sequencing and reverse transcriptase PCR were performed on two ganglia from the crab Cancer borealisa model in which the identified neurons had previously been classified. The team essentially worked backward from previous results to validate the use of RNA sequencing.

Schulz explained, “with known cell identity to work from, we could blind ourselves to their identities for some of the analyses and see how well unbiased statistical methods perform in terms of recapitulating known biologically/physiologically known cell identity.”



Each neuron analyzed exhibited distinct gene expression patterns; however, expression profiles were not enough to accurately identify the neurons. Unbiased clustering analysis and supervised machine learning were also integrated into this approach.

The results demonstrated that transcriptional profiling for the assignment of neuronal identity is most accurate when combined with information regarding the physiology and morphology of the cells.

Identifying and understanding the operation of a single neuron has implications for developing targeted therapies for neurodegenerative diseases. RNA sequencing has the potential to inform our knowledge of how neurons are affected by injury and disease.

Understanding the intersect between variability, environment and genetics could advance insight into how individuals are differently affected by neurological diseases, contributing to the precision medicine revolution.

The team now hopes to utilize the knowledge gained from this study to determine how neurons are affected in spinal cord injuries.

“We are working to understand how injuries and diseases that cause changes to the normal activity of neural networks affect the neuronal gene expression profiles, and what this can tell us about how even indirect effects can contribute to pathology “downstream” of the actual insult,” concluded  Schulz.

If you were interested in this post and would like to learn more, check out the Cells as Models track of our Online Event: Advancing Precision Medicine 2019  and register for free today. To get more information visit the event page here.

WRITTEN BYCaitlin Killen

SOURCENorthcutt AJ, Kick DR, Otopalik AG et al. Molecular profiling of single neurons of known identity in two ganglia from the crab Cancer borealis. Proc. Natl. Acad. Sci. USA. doi: 10.1073/pnas.1911413116 (2019);https://www.pnas.org/content/early/2019/12/04/1911413116https://news.missouri.edu/2019/think-like-a-crab/

RELATED TAGS

NEURAL NETWORKSNEURONSRNA SEQUENCINGSHAREREFER A COLLEAGUEPREVIOUS ARTICLENEXT ARTICLE

Related articles

 

 

 

 

 

 

 

 

 

Your subscriptions

BioTechniques is a must have resource for anyone working or studying in the life sciences field providing the latest methods, techniques and protocols – Subscribe for FREE today!SIGN UPMANAGE
To unsubscribe please click the button belowUNSUBSCRIBE

OFFICE INFO

BIOTECHNIQUES is powered by Future Science Group.
Unitec House, 2 Albert Place, London, N3 1QB, UK
+44 (0)20 8371 6090STAY CONNECTEDtwitterfacebooklinkedinrssFUTURE SCIENCE GROUP

© 2019 BIOTECHNIQUES

 
 
 

RNA

From Wikipedia, the free encyclopedia
 
 
Jump to navigationJump to search

RNA is an acronym for ribonucleic acid, a nucleic acid. Many different kinds are now known.[1]

RNA is physically different from DNA: DNA contains two intercoiled strands, but RNA only contains one single strand. RNA also contains different bases from DNA. These bases are the following:

(A) Adenine
(G) Guanine
(C) Cytosine
(U) Uracil

Adenine forms bonds with uracil, and guanine forms bonds with cytosine. In this way, we say that adenine is complementary to uracil and that guanine is complementary to cytosine. The first three bases are also found in DNA, but uracil replaces thymine as a complement to adenine.

RNA also contains ribose as opposed to deoxyribose found in DNA. These differences result in RNA being chemically more reactive than DNA. This makes it the more suitable molecule to take part in cell reactions.

RNA is the carrier of genetic information in certain viruses, especially the retroviruses like the HIV virus. This is the only exception to the general rule that DNA is the hereditary substance.

Protein synthesis RNAs[change | change source]

Messenger RNA[change | change source]

 
The structure of a mature eukaryotic mRNA. A fully processed mRNA includes a 5′ cap5′ UTRcoding region3′ UTR, and poly(A) tail. UTR = untranslated region

The main function of RNA is to carry information of amino acid sequence from the genes to where proteins are assembled on ribosomes in the cytoplasm.

This is done by messenger RNA (mRNA). A single strand of DNA is the blueprint for the mRNA which is transcribed from that DNA strand. The sequence of base pairs is transcribed from DNA by an enzyme called RNA polymerase. Then the mRNA moves from the nucleus to the ribosomes in the cytoplasm to form proteins. The mRNA translates the sequence of base pairs into a sequence of amino acids to form proteins. This process is called translation.

DNA does not leave the nucleus for various reasons. DNA is a very long molecule, and is bound in with proteins, called histones, in the chromosomes. mRNA, on the other hand is able to move and to react with various cell enzymes. Once transcribed, the mRNA leaves the nucleus and moves to the ribosomes.

Two kinds of non-coding RNAs help in the process of building proteins in the cell. They are transfer RNA (tRNA) and ribosomal RNA (rRNA).

tRNA[change | change source]

Transfer RNA (tRNA) is a short molecule of about 80 nucleotides which carries a specific amino acid to the polypeptide chain at a ribosome. There is a different tRNA for each amino acid. Each one has a site for the amino acid to attach, and an anti-codon to match the codon on the mRNA. For example, codons UUU or UUC code for the amino acid phenylalanine.

rRNA[change | change source]

Ribosomal RNA (rRNA) is the catalytic component of the ribosomes. Eukaryotic ribosomes contain four different rRNA molecules: 18S, 5.8S, 28S and 5S rRNA. Three of the rRNA molecules are synthesized in the nucleolus, and one is synthesized elsewhere. In the cytoplasm, ribosomal RNA and protein combine to form a nucleoprotein called a ribosome. The ribosome binds mRNA and carries out protein synthesis. Several ribosomes may be attached to a single mRNA at any time.[2] rRNA is extremely abundant and makes up 80% of the 10 mg/ml RNA found in a typical eukaryotic cytoplasm.[3]

snRNAs[change | change source]

Small nuclear RNAs (snRNA) join with proteins to form spliceosomes. The spliceosomes govern alternative splicing. Genes code for proteins in bits called exons. The bits can be joined together in different ways to make different mRNAs. Thus, from one gene many proteins can be made. This is the process of alternative splicing. Any unwanted versions of the protein get chopped up by proteases, and the chemical bits re-used.

Regulatory RNAs[change | change source]

There are a number of RNAs which regulate genes, that is, they regulate the rate at which genes are transcribed or translated.[4]

miRNA[change | change source]

Micro RNAs (miRNA) act by joining an enzyme and blocking mRNA, or speeding its breakdown. This is called RNA interference.[5][6][7]

siRNA[change | change source]

Small interfering RNAs (sometimes called silencing RNAs) interfere with the expression of a specific gene. They are quite small (20/25 nucleotides) double-stranded molecules. Their discovery has caused a surge in biomedical research and drug development.[8][9]

Parasitic and other RNAs[change | change source]

Retrotransposons[change | change source]

Transposons are only one of several types of mobile genetic elementsRetrotransposons copy themselves in two stages: first from DNA to RNA by transcription, then from RNA back to DNA by reverse transcription. The DNA copy is then inserted into the genome in a new position. Retrotransposons behave very similarly to retroviruses, such as HIV.

Viral genomes[change | change source]

Viral genomes, which are usually RNA, take over the cell machinery and make both new viral RNA and the protein coat of the virus.

Phage genomes[change | change source]

Phage genomes are quite varied. The genetic material can be ssRNA (single-stranded RNA), dsRNA (double-stranded RNA), ssDNA (single-stranded DNA), or dsDNA (double-stranded DNA). It may be between 5 and 500 kilo base pairs long with either circular or linear arrangement. Bacteriophages are usually between 20 and 200 nanometers in size.

Phage genomes may code for as few as four genes,[10] and as many as hundreds of genes.

References[change | change source]

  1.  Only the most important are described here. A complete list is available at en:List of RNAs.
  2.  Cooper GC & Hausman RE (2004). The Cell: a molecular approach (3rd ed.). Sinauer. pp. 261–76, 297, 339–44. ISBN 0-87893-214-3OCLC 174924833 52121379 52359301 56050609 Check |oclc= value (help).
  3.  Kampers T.; et al. (1996). “RNA stimulates aggregation of microtubule-associated protein tau into Alzheimer-like paired helical filaments”. FEBS Letters399 (3): 104D. doi:10.1016/S0014-5793(96)01386-5PMID 8985176. Explicit use of et al. in: |author= (help)
  4.  Morris KV (2008). http://www.horizonpress.com/rnareg |chapterurl= missing title (help)RNA and the regulation of gene expression: a hidden layer of complexity. Caister Academic Press. ISBN 978-1-904455-25-7.
  5.  The Nobel Prize in Physiology or Medicine 2006. RNA Interference
  6.  Lee R.C. & Ambros V. 2001. An extensive class of small RNAs in Caenorhabditis elegans. Science 294, 862-864.
  7.  Lau N.C. et al 2001. An abundant class of tiny RNAs with probable regulatory roles in Caenorhabditis elegans. Science 294, 858-862.
  8.  Hamilton A. & Baulcombe D (1999). “A species of small antisense RNA in posttranscriptional gene silencing in plants”. Science286: 950–2. doi:10.1126/science.286.5441.950PMID 10542148. First description of siRNAs.
  9.  Hannon G. & Rossi J (2004). “Unlocking the potential of the human genome with RNA interference”Nature431: 371–8. doi:10.1038/nature02870PMID 15372045.
  10.  Bacteriophage MS2
 
 

8 Comments

  1. keep up the wonderful work, I read few articles on this website and I believe that your web site is rattling interesting and contains lots of good info .

    Like

Leave a Reply

Fill in your details below or click an icon to log in:

WordPress.com Logo

You are commenting using your WordPress.com account. Log Out /  Change )

Google photo

You are commenting using your Google account. Log Out /  Change )

Twitter picture

You are commenting using your Twitter account. Log Out /  Change )

Facebook photo

You are commenting using your Facebook account. Log Out /  Change )

Connecting to %s

transbiotex.wordpress.com/

Personal Cellular Storage for Regenerative Medicine

WordPress.com em Português (Brasil)

As últimas notícias do WordPress.com e da comunidade WordPress

%d bloggers like this: